FORMULAE
Absolute leukocyte count = Differential Leukocyte Count % x TLC
Body Surface Area (Mosteller formula) in square metre = under root (height in cm x weight in kg / 3600)
Corrected Calcium = S. Calcium mg/dL + [0.8 x (Normal Albumin - Patient's Albumin)]
in other words, each 1 g/dL decrease of albumin will decrease 0.8 mg/dL in measured serum Ca and thus 0.8 must be added to the measured Calcium to get a corrected Calcium value.
Calcium in mg/dL = 4 x Calcium in mmol/L
Citrate needed according to high haematocrit = 0.00185 x (100 - Hct) x volume of blood to be added
Corrected Creatinine Clearance = (Urinary creatinine/Plasma creatinine x Volume of urine in mL per minute) x (1.73/Body Surface Area)
Corrected reticulocytes count (Reticulocytes Index) = (Patient's Hct/Normal Hct) x Reticulocyte %
Corrected TLC = [100 / (100+NRBC)] x TLC
Fall in Serum Ferritin per venesection = 25 ng/mL
Iron requirement = (repletion of iron stores) + (repletion of circulating iron)
= (1000 mg) + (weight in kg x Desired increment in Hb in g/dL x 2.2)
Malarial Parasite Index (Thin smear) = Number of Trophozoites & Schizonts in 1000 RBCs / 10
Malarial Parasite Density (Thin smear) = Number of Trophozoites & Schizonts in 1000 RBCs x Analyser count RBC per microlitre / 1000
One drop of blood = 50 uL
Manual Platelet count using peripheral blood smear (in 10^3/uL)=
Indirect Method 1 (more accurate)=(Number of platelets per 1000 RBC) x TRBC x 1000
Indirect Method 2 (more easy)=(Number of platelets in a field having 50% red cells in triplets using Oil immersion lens) x 10 fields/10 x Multiplication Factor eg 10,000
Note: Indirect method 2 needs verification of the Multiplication Factor according to the Field Diametre of the microscope
Manual WBC count using peripheral blood smear (in 10^3/uL)= Number of WBCs in 10 fields using 40x objective/10 x 3.5 as Multiplication factor
Note: Multiplication factor is the ratio of count on blood smear at 40x objective versus count on analyser.
Field Diametre (Area of the field seen under a microscope)= Field number of eye piece/Total magnification
rpm = under root [ (g x 105) / (1.118 x radius of rotor) ]
Red cell replacement in children
= Desired increment in Hb in g/dL x Weight in kg x 3/Hct of the blood bag
Reticulocyte Proliferation Index = Reticulocyte Index / Correction factor
[Note: Correction factor=1.5 if Hct 35 L/L, 2 if Hct 25 L/L, 2.5 if Hct 15 L/L]
Stem cell dose = Absolute mononuclear cells in peripheral blood x Amount of harvest in mL / recipient's weight in kg
Total leukocyte count = [Average number of WBC in 10 fields using x40 objective in morphology area] x 3